320-022125 Focus 25.1

Focus Mar. 2003 Volume 25.1 3 incorporation into product during PCR. The design is based on the effects of the primary and secondary structure of oligonucleotides on the emission properties of attached fluorescent dyes (6). The design factors are used in a proprietary primer design software, called LUXTM Designer, that is used to design and order fluorogenic LUXTM Primers and unlabeled counterparts against the gene or sequence target of interest. The software can be accessed in three ways, as a desktop format that can be downloaded and installed on your computer, as a webbased primer design and ordering tool at http://www.invitrogen.com/lux, or by request from Invitrogen.